Home Ozonoterapia y enfermedades infecciosas El ozono inactiva el VIH a concentraciones no citotóxicas

El ozono inactiva el VIH a concentraciones no citotóxicas

Michael T. F. Carpendale and Joel K. Freeberg


 Rehabilitation Medicine and Research Services, Veterans Administration Hospital, San Francisco, CA 94121, U.S.A.


 Department of Orthopaedic Surgery, University of California School of Medicine, San Francisco, CA 94143, U.S.A.


Center for Medical Ozone Studies, Bay Medical Research Foundation, San Francisco, CA 94117, U.S.A.

Received 8 October 1990; 
accepted 27 April 1991. 
Available online 12 November 2002. 
 

Abstract

The inactivation of human immunodeficiency virus (HIV) and cytotoxic properties of ozone-treated serum and serum-supplemented media were examined. The titer of HIV suspensions in human serum was reduced in a dose-dependent manner when treated with total reacted ozone concentrations at a range of 0.5 to 3.5 μg/ml−1. Complete inactivation of HIV suspensions was achieved by 4.0 μg/ml−1 of ozone in the presence or absence of H-9 cells. In contrast, cellular metabolism, as measured by MTT dye cleavage, and DNA replication, as measured by BUdR incorporation, were enhanced in H-9 cells grown in media treated with quantities of ozone that completely inactivate HIV. The permissively HIV-infected cell line HXB/H-9 was cultured in ozone-treated media for six days with culture supernatants being sampled and assayed on alternate days for HIV p24 core protein. HIV p24 was reduced in all treated cultures compared to control cultures, with an average reduction of 46% [p24].

Author Keywords: HIV; Ozone; Noncytotoxic; Virus inhibitor

Ultima actualización (Domingo 04 de Abril de 2010 21:28)